This study aimed to examine the extent to which institutional excellence is practiced in enhancing the performance of employees at the Ministry of Education in the Gaza Strip, Palestine. The study sample comprised 130 employees occupying supervisory positions within the Ministry. Given the nature of the research, the descriptive–analytical approach was employed. The findings indicated that the overall level of institutional excellence practice in enhancing employee performance was high, with a relative weight of 80.2%. The knowledge excellence dimension, as one of the core institutional excellence criteria, also achieved a high level of practice, recording a relative weight of 77.4%. Similarly, the leadership excellence dimension demonstrated a high level of implementation, with a relative weight of 82.5%, while service excellence likewise attained a high level, with a relative weight of 80.6%. The statistical analysis further revealed significant differences in respondents’ perceptions of the degree of institutional excellence practice attributable to gender, with female respondents reporting significantly higher evaluations than their male counterparts. In contrast, no statistically significant differences were identified with respect to educational qualification or years of service. Based on these findings, the study recommends fostering a supportive organizational culture that promotes service quality and institutional distinction by creating an organizational climate grounded in shared values, principles, and beliefs that enhance employees’ capabilities and professional effectiveness. The Ministry should also encourage the adoption of innovative ideas, invest in creative behaviors, and establish mechanisms for nurturing innovation as a strategic driver of institutional excellence. Furthermore, employees at all administrative levels should be actively involved in organizational change, development, and continuous improvement initiatives through management practices that facilitate successful change implementation, including delegated authority, participatory decision-making, and other collaborative approaches, thereby strengthening institutional excellence across the Ministry of Education in the Gaza Strip. Keywords: Institutional Excellence - Ministry of Education - Southern Palestinian Governorates