Aloe Vera has gained significant popularity in both traditional and official medicinal practices. Traditionally, the gel produced by the plant has been used to heal burns and wounds, among other conditions. The formulation and characterisation of an in-situ gel formulation containing aloe vera extract for topical application are the primary objectives of the current study. Carbopol 934 (CP934), hydroxy propyl methyl cellulose (HPMC), and Pluronic F127 (Pl-F127) were all employed in the cold method formulation of the in-situ gel. A total of eight distinct formulations were created, each varying in the ratio and type of polymers that form the hydrogel. The evaluations of all formulations included the analysis of their appearance, consistency, homogeneity, transparency, and the temperature at which gelation occurs. Based on the established evaluation parameters, the ideal formulations underwent additional assessment concerning pH measurement, spreadability, viscosity, gel strength, gelling capacity, skin irritation tests, and in-vitro antibacterial assay. The evaluation study's findings suggest that the AVg1 formula is a good herbal alternative for treating bacterial infections that occur topically. ............. Keywords:............... Aloe Vera, In-Situ Gel, Wound healing, Physicochemical properties, Antibacterial assay.