The extraction technique is very critical step in studying the biological activity of medicinal plants; it considerably influences the study's final outcome of the experiment. In order to have proper extraction procedure, it is essential to choice an, cheaper, simpler, , efficient ,quicker, and ecologically friendly way and to get the selected bioactive constituents with greater effectiveness. The aim of this study was to compare between maceration and soxhlet procedures in studying the antibacterial effect of Rosemary leaves. Using hexane and methanol respectively as solvents, the data of % of yield extracts showed that soxhlet was have higher yield than maceration, on the other hand, extracts by maceration methods displayed higher antibacterial activity, this could be attributed to that some biological active compounds are heat thermo labile, but still soxhlet consider a good alternative extraction method. In conclusion, maceration extraction is more effective for obtaining extracts with strong antibacterial activity from rosemary leaves. Future research should check Rosemary leaf extracts against a wider range of bacteria, including Gram-positive, Gram-negative, and resistant strains, for a completing understanding of their antibacterial properties............... Keywords:................. Maceration, Soxhlet, Rosemary, Extraction, Antibacterial.