Background: Iron and Folic acid are essential micronutrients for reproductive health. Although dietary supplements are common during pregnancy, excessive intake can impair liver and kidney function. Aim: To study the effect of Iron and Folic acid supplements and overdoses on the hematopoietic function, liver functions and renal functions of female mice. Methodology: The animals were raised and provided with appropriate conditions for reproduction in an animal house at the Faculty of Veterinary Medicine, University of Tripoli, until the required number for the experiment was obtained. The doses were given to 36 female mice only, which were divided into 6 groups: the control group and 5 other groups were divided as follows: Group I (control), Group II (Fe 15mg/kg), Group III (FA 8mg/kg), and Group IV (FA 4mg/kg). Group V (Fe with FA 8mg/kg) and Group VI (Fe with FA 4mg/kg). Every other day intraperitoneal injections of Fe were administered, and oral FA supplements were provided daily for a week, mice were monitored and weighed weekly and fed daily for 3 weeks. Liver and kidney enzyme levels were measured, and blood component levels were assessed. Data were analysed using analysis of variance (ANOVA). Results: The effect of treatments on liver and kidney functions was analysed using one-way analysis of variance (ANOVA). The results showed that Iron and Folic acid supplementation had an impact on liver and kidney functions. Liver enzymes in female rats supplemented with Iron showed a significant increase (p < 0.05) compared to female rats supplemented with Folic acid alone or with Iron, which led to a significant increase in liver enzymes and serum creatinine levels, and worsened kidney function. Although liver enzymes increased after supplementing with Iron alone (group II), Folic acid alone (group III), but after treatment with both (group V and VI), the increase in liver enzymes was significantly lower. Conclusion: Excess Iron and Folic acid negatively affect female health, with high Iron doses potentially increase the risk of the liver and kidney. This research highlights the importance of screening of Iron levels before supplementation and raises awareness about the risks of unnecessary Iron intake.................... KEYWORDS:................ Folic acid, Iron toxicity, anemia, supplementation, Liver, Kidney.