The widespread zoonotic parasite Toxoplasma gondii has serious consequences for public health, especially for the health of mothers and newborns. The impact of climatic conditions on acute infection is yet unknown, despite numerous reports of high seroprevalence in Libya. 168 serum samples were obtained from Al-Saleem Laboratory in Benghazi, Libya, between November 2024 and November 2025 for this cross-sectional investigation, and they were tested for IgM antibodies. At the same time, serological results were combined with meteorological data on humidity and temperature. The total IgM prevalence was 5.95%, and there was clear seasonal clustering, with 80% of cases occurring between August and November. IgM positivity showed a positive correlation with humidity (ρ = 0.18, P = 0.019) and temperature (ρ = 0.21, P = 0.007).and logistic regression indicated that both are independent predictors, with each 1°C increase in temperature and 1% rise in humidity increasing the probabilities of IgM positive by 19% and 13%, respectively. These findings provide the first evidence from Libya relating acute toxoplasmosis to climate variability, emphasizing the importance of environmental factors in parasite transmission. This study contributes to addressing a significant research gap by providing a solid framework to guide public health interventions in the city of Benghazi, Libya................... Keywords:............... Toxoplasma gondii, Acute toxoplasmosis, IgM seropositivity, Environmental factors , Benghazi, Libya