Lead is a heavy metal known for its toxicity effect, which causes many effects on different organs of the body, and lead acetate is a form of lead in the environment. Due to the health and protective nature of the olive tree, by its elements, against many diseases, the leaf extract of this tree was chosen in our study because it is rich in oleuropein and hydroxytyrosol. The experiment was conducted in a special farm in the Al-Qubbah region in eastern Libya during November and December 2024. This study was to investigate the role of olive leaf (Olea europea L.) extract (OLE) against lead acetate (PbAc) effect on liver enzyme activity (AST, ALT, and LDH) in adult male rabbits. Sixteen adult male rabbits were divided into four groups: untreated as a control (G1), treated with PbAc (G2), treated with OLE (G3), and treated with a mixture of PbAc + OLE (G4). The results showed that the use of lead acetate led to an increase in liver enzyme activity compared to the control group. However, the use of an aqueous extract of olive leaves improved enzyme activity, whether used alone or in combination with lead acetate. Based on these results, it can be concluded that olive leaf extract (OLE) has protective and therapeutic effects against the negative impact of lead acetate on the activity of the liver enzymes under study (AST, ALT, and LDH)................. Keyword:............... Lead, Pb, plant extracts, olive, rabbits, AST, ALT, LDH, liver enzymes