Emulsification is one of the most persistent operational challenges in the petroleum industry, adversely affecting crude oil production, transportation, and processing. A thorough understanding of emulsion formation and stability is essential for developing efficient demulsification strategies that minimize corrosion, viscosity increase, and flow assurance problems. This study investigates the formation of water-in-oil emulsions in crude oil flow lines and evaluates the performance of different demulsification agents, including organic solvents, commercial demulsifiers, polymers, and a natural material (MLX), as environmentally friendly alternatives. Centrifuge experiments were conducted using two crude oil types (M.F. and S.F.) with different API gravities. All tests were performed at 1500 rpm, 60 °C, and a centrifugation time of 10 minutes, using additive concentrations ranging from 0.25% to 1%. Separation efficiency was evaluated based on the volume of separated water and the reduction in emulsion layer thickness. The results indicate that commercial demulsifiers achieved the highest separation efficiency, particularly for the lighter crude oil (M.F.). Organic solvents such as xylene and hexane showed moderate effectiveness, with performance strongly dependent on crude oil properties. Polymer-based treatments exhibited limited efficiency, especially for the heavier crude oil (S.F.). Notably, the natural material MLX demonstrated a clear concentration-dependent improvement in demulsification performance. Increasing the MLX concentration to 0.5% and 1% significantly enhanced water separation and reduced the emulsion layer in both crude oil samples. These findings confirm the potential of MLX as a promising natural and sustainable demulsification agent and highlight its applicability as an effective alternative or supplement to conventional chemical demulsifiers in crude oil processing............... Keywords: ...............crude oil emulsion, demulsification, natural demulsifier, centrifugal separation, green chemicals.