Paper currencies is not limited to the banknotes themselves; it also includes surfaces that carry numerous invisible microbes, posing a potential public health concern. This study aimed to assess bacterial and fungal contamination of paper currency circulating in butcher shops and poultry stores in the city of Zawia - Libya, as well as to identify the most contaminated denominations. Seventy-five samples of circulating banknotes were collected from five stores, with five samples of each denomination (1, 5, and 10 Libyan dinars). The study was conducted in the Microbiology Laboratory of the Faculty of Science, Department of Botany. Bacterial and fungal growth was monitored, and pure cultures were obtained. Growing colonies were identified based on their morphological characteristics and the cells' response to biochemical tests. The results showed that 73% of harmful viruses contaminated with bacteria and fungi were removed. Nineteen fungal isolates and 22 bacterial isolates belonging to six fungal genera (Alternaria spp., Fusarium spp., Cladosporium spp., Aspergillus spp., Rhizopus spp., and Saccharomyces spp.) and seven bacterial genera (Micrococcus spp., Staphylococcus spp., Klebsiella spp., Shigella spp., Salmonella spp., Providencia spp., and Pseudomonas spp.) were removed. This study recommends to separate the handling of mobile phones from meat by using secure collection boxes or electronic payment methods, and to promote regular handwashing and surface disinfection. It also emphasizes the importance of raising consumer awareness about the transmission of microbes through clean currency.................. Keywords:................Pathogenic bacteria, microbial contamination, Clean currency, Fungi, Zawia city.