Computer peripherals, particularly keyboards and mice, were among the most frequently touched surfaces in academic and shared environments and were rarely subjected to routine disinfection. This study aimed to investigate the microbiological contamination of these multi-user devices in the administrative offices of the Faculty of Science at Omar Al-Mukhtar University, to identify the bacterial isolates present, and to evaluate their antibiotic resistance profiles. A total of 160 swab samples were collected from keyboards and mice and cultured on selective and differential media, including Blood Agar, Mannitol Salt Agar, MacConkey Agar, and CzapekDox Agar. Microbial growth was observed on 56 plates, yielding 78 bacterial isolates. Gram staining revealed that 84.6% were Gram-positive and 15.4% were Gram-negative. No fungal growth was detected on CzapekDox Agar. The identified bacterial species included Staphylococcus hominis, Staphylococcus lugdunensis, Staphylococcus capitis, Pseudomonas stutzeri, Pantoea spp., Klebsiellaoxytoca, Escherichia coli, Citrobacter spp., and Pseudomonas aeruginosa. Antimicrobial susceptibility testing revealed varying patterns of resistance, with amoxicillin-clavulanic acid (50%) and cefoxitin (62.5%) showing the highest resistance rates. Notably, all isolates remained completely susceptible to imipenem, ceftazidime, levofloxacin, gentamicin, and ciprofloxacin. The results highlighted the critical need for improved hygiene practices by demonstrating that computer peripherals could serve as potential reservoirs for pathogenic bacteria................. Keywords: ................keyboard, mouse, pathogen bacteria, administrative offices, multi-user.