Milk quality and nutritional value are strongly influenced by its physicochemical properties and chemical composition, which vary among animal species. This study aimed to comparatively evaluate the physicochemical characteristics and chemical composition of fresh raw cow, goat, and sheep milk collected from farms in Tocra, Libya. A total of 30 raw milk samples (10 from each animal species) were collected during the spring season. The samples were analyzed for pH, titratable acidity (expressed as lactic acid), protein content, ash content, and starch adulteration, total solids (TS). The measured values ranged from 0.13 to 0.20% for titratable acidity, 6.57 to 6.75 for pH, 2.96 to 6.20% for protein, 0.62 to 1.05% for ashe, and 11.14 to 16.90% for TS. No starch adulteration was detected in any of the samples analyzed. The results demonstrated species-dependent variations in the physicochemical properties and chemical composition of the milk samples, reflecting differences in their nutritional characteristics. These findings provide baseline information on the quality of fresh milk produced in the Tocra region and may contribute to quality assessment and future dairy research in Libya. Further microbiological and storage studies are recommended to evaluate the shelf-life stability of these milk samples. The higher protein, ash, and titratable acidity values observed in sheep milk highlight its favorable compositional characteristics for cheese production.................. Keywords: ............Raw milk, Physicochemical analysis, Milk quality, Titratable acidity, Protein content, Ash.