Polyaniline Nanocomposites is very important to understand and evaluate the mechanical, thermal, and chemical stability properties of the nanocomposite materials. Polyaniline/layered Titanosilicate (PANI/JDF-L1) nanocomposites were prepared by in situ polymerization of anilinium monomer with layers of (Na+- JDF-L1) using ammonium peroxy disulfate (APS) as an oxidizing agent. Different amounts of aniline were used to verify this effect on the characteristics of the formed nanocomposites. The prepared PANI/JDF-L1 nanocomposites were characterized by UV-VIS, Fourier Transform Infrared (FT-IR), X- ray diffraction (XRD), Thermo Gravimetric Analysis (TGA), and conductivity measurement. The formation of nanocomposites was confirmed by FT-IR, UV-VIS. The basal spaces of PANI/JDF-L1 nanocomposites which decreased after the polymerization process as evidenced from X-ray diffraction (XRD). At a higher level of loading the polyaniline, layered structure of nanocomposite breaks forming exfoliated nanocomposite, revealing well-defined nanosized morphology of polyaniline Titanium Silicate (JDF-L1).The PANI/JDF-L1 nanocomposite was found to be thermally stable up to 340Co and showed conductivity value 4.9 × 10-4 S/cm with 13.4 wt % loading of PANI-ES in JDF-L1.