In Libya, there are multiple sources of pollution, one of which is animal waste. The anaerobic digestion (AD) of organic wastes to produce biogas has the advantage of producing valuable, renewable energy while reducing the environmental impact of these wastes. Cowmanure have the potential to produce biogas due to their high organic content. This study aimed to study different concentrations for the feedstock (1:1 and 2:1 cow manure: water v/v) to monitor which one gives higher biogas production. A plastic tank with a capacity of 72 liters and a feedstock volume of 60 liters was used to create a pilot scale. The biogas was analyzed using a GC device at the end of the experiment in the Zawiya Oil Refining Company. The result indicated that the ratio of 2:1 resulted in a biogas production increase of 8.3% more than 1:1, with a yield of 630 g/day for 16 days. Also, the mixture of 2:1 contained 78.71% of methane, while the mixture of 1:1 contained 58% of methane. Keywords: Biogas, Cow manure, Anaerobic digestion and Renewable energy.